State Road 64 travels from west to east through the counties of Manatee, Hardee and Highlands.

SR 64 begins at Gulf Drive (City Road 789) in Holmes Beach on Anna Maria Island and heads east along Manatee Avenue.

It crosses the Braden River and exits Bradenton city limits before coming to an interchange with Interstate 75 (I-75).

It then passes to the north of Wingate Creek State Park and through Myakka Head before entering Hardee County.

In Ona, it crosses CSX's Brewster Subdivision and intersects CR 663, which runs north to Fort Green and south to Limestone and Arcadia.

[6] Perico Island was previously access via a road that ran from 9th Avenue North southwest along the present-day Spoonbill Trail.

County Road 64 (CR 64) is the unsigned designation for 75th Street Northwest on the west side of Bradenton.

After crossing a bridge over Bonnet Creek, it crosses the Polk County Line[13] thus entering part of the Lake Wales Ridge State Forest, before curving east as it approaches a bridge over Arbuckle Creek, and terminating at the Avon Park Air Force Range.

By the time it reaches the intersection of CR 35B, it runs directly west and east like its parent route and enters the City of Wauchula.