[6] In temperate Europe, flufenacet is commonly used in cereal crops to control grass weeds such as black-grass (Alopecurus myosuroides) that have evolved resistance to other herbicides and is applied in combination with other herbicides including pendimethalin, diflufenican, flurtamone, metribuzin and aclonifen.

[7] As of 2019[update] in the United Kingdom, resistance to flufenacet had began to be found in black-grass and rye-grass.

[10][11] In September 2024, the European Food Safety Authority has concluded that flufenacet is an endocrine disruptor of humans and wild mammals via the thyroid (T)-modality.

[10] The active substance falls under the exclusion criteria of the Plant Protection Products Regulation.

[12][13] Flufenacet can be obtained by reacting equivalent amounts of 2-methylsulfonyl-5-trifluoromethyl-1,3,4-thiadiazole with 2-hydroxy[N-(4-fluoropheny)-N-isopropyl]acetamide in the presence of sodium hydroxide in acetone.