Gabriel de la Cueva y Girón, 5th Duke of Alburquerque, 2nd Marquess of Cuéllar, 5th Count of Ledesma, 5th Count of Huelma (c. 1515 – 1571) was a Spanish nobleman and military leader who served as Viceroy of Navarre from 1560 to 1564 and Governor of Milan from 1564 to his death in 1571.

He was born in Cuéllar, the son of Don Beltrán de la Cueva, 3rd Duke of Alburquerque and of Doña Isabel Girón, and inherited the title from his older brother, Francisco Fernández de la Cueva, 4th Duke of Alburquerque.

After leading in 1556 the defense of Oran against the Turks,[1] Gabriel de la Cueva was appointed Viceroy of Navarre in 1560, to replace his father who had died, and later Governor of the Duchy of Milan in 1564, a position that he held until his own death in 1571 Correction to the above paragraph: (Ayes, sic, Gabriel de la Cueva y Giron was the son of don Beltrán de la Cueva, III Duke of Alburquerque and of Doña Isabel Téllez Girón and inherited the title from his grandfather, Francisco Fernández de la Cueva y Mendoza II Duke of Alburquerque and his wife, Francisca Álvarez de Toledo.

Source of proof, Ministry of Culture Spain: Section of Nobility of the National Historical Archive: Archivo: Sección Nobleza del Archivo Histórico Nacional Signatura: OSUNA,C.4,D.1-2 Código de Referencia:

Translation, ( Ayes, sic: Record of matrimony between Francisco Fernández, de la Cueva, II Duke of Alburquerque and Juan Téllez-Girón, 2nd Count of Ureña, fathers of Beltrán de la Cueva, III Duke of Alburquerque and Isabel Girón, in the marriage of their children.

- Cédula de la reina Juana dando licencia para el casamiento.

During his reign in Milan, Doña Juana received from Pope Pius V the Golden Rose, normally only given to Kings and Emperors.

They had two daughters : Gabriel was succeeded by his cousin Beltrán de la Cueva.