Genotyping by sequencing

[2] Although GBS presents an approach similar to restriction-site-associated DNA sequencing (RAD-seq) method, they differ in some substantial ways.

[1] In summary, high molecular weight DNAs are extracted and digested using a specific RE previously defined by cutting frequently[7] in the major repetitive fraction of the genome.

Once a large-scale, species-wide SNP production has been run, it is possible to quickly call known SNPs in newly sequenced samples.

[8] When initially developed, the GBS approach was tested and validated in recombinant inbred lines (RILs) from a high-resolution maize mapping population (IBM) and doubled haploid (DH) barley lines from the Oregon Wolfe Barley (OWB) mapping population.

Up to 96 RE (ApeKI)-digested DNA samples were pooled and processed simultaneously during the GBS library construction, which was checked on a Genome Analyzer II (Illumina, Inc.).