A glovebox (or glove box) is a sealed container that is designed to allow one to manipulate objects where a separate atmosphere is desired.

A smaller antechamber compartment is used to transport items into or out of the main chamber without compromising the internal environment.

Copper metal (or some other finely divided metal) is commonly used to remove oxygen, this oxygen removing column is normally regenerated by passing a hydrogen/nitrogen mixture through it while it is heated: the water formed is passed out of the box with the excess hydrogen and nitrogen.

Also, coordinating solvents, such as tetrahydrofuran and dichloromethane, can bind irreversibly to the copper catalyst, reducing its effectiveness.

At the now-deactivated Rocky Flats Plant, which manufactured plutonium triggers, also called "pits", production facilities consisted of linked stainless steel gloveboxes up to 64 feet, or 20 meters, in length, which contained the equipment which forged and machined the trigger parts.