Glycopeptide

[3][4][5] The synthesis of glycopeptides provides biological probes for researchers to elucidate glycan function in nature and products that have useful therapeutic and biotechnological applications.

Although the majority of N-linked glycans take the form GlcNAc-β-Asn[6] other less common structural linkages such as GlcNac-α-Asn[7] and Glc-Asn[8] have been observed.

This approach is not as popular as the linear strategy due to the poor reaction yields in the glycosylation step.

[18] In this method, libraries of glycopeptides are produced on a cellulose surface (e.g. filter paper) which acts as the solid phase.

The scale of this technique can be an advantage for creating libraries for screening by using less amounts of glycoamino acids per peptide.

Native chemical ligation (NCL) is a convergent synthetic strategy based on the linear coupling of glycopeptide fragments.

Unlike standard SPPS (which is limited to 50 amino acid residue) NCL allows the construction of large glycopeptides.