The player controls Juan Aguacate, a luchador who fights with punches, kicks, and wrestling grapples.

He also learns new tricks from completing challenges set by trainers found throughout the world, such as more damaging attacks or additional health.

The most drastic change is the chicken transformation - while in the previous game it was only for traversing small corridors, it now has an entire fighting moveset of its own with unique special moves.

In this timeline, Juan and Lupita were killed by Calaca, who was defeated by another luchador called Salvador.

In the seven years since, Salvador has become corrupted by the power of his mask, and now wishes to collect three relics alongside Salvador’s assistants: sociopathic musician and playwright El Muneco, the murderous, cruel anthropomorphic and sentient dog-skeleton Uay Pek, and a hyper intelligent vulture and cactus named Zope and Cactuardo.

Salvador plots to access the Sacred Guacamole in the realm of El Otromundo and become all-powerful.

In the true ending, attained if the player clears the Chicken Illuminati's crucible and meets the Holy Hen, Juan recalls her advice and removes his mask, immediately identifying the correct portal and returning to his family without delay.

The first downloadable content called Three Enemigos Pack, was released on November 27, 2018 and includes three new character costumes: El Muñeco, Uay Pek and Jaguar Javier who also appear as bosses in the main game.

After obtaining a gold medal for all challenges in the group the player is rewarded with a costume of the trainer; if the player obtains gold medals for all 15 challenges, they are granted access to the Salvador costume and can play as the main antagonist of the game.

[6] Eliot Osage of Bonus Stage commented on the difficulty of the challenges calling them "punishing" and also stating "The Proving Grounds will ask you to use every bit of skill, cunning and patience that you’ve developed throughout your play of Guacamelee 2 [sic] in order to complete them" and ultimately said that it is "a truly challenging bonus world with tantalizing rewards".

2, the studio was able to revisit ideas that were scrapped during the development of the first game, and expand on the existing mechanics and systems.