Barbara Anne "Babs" Bunny (voiced by Tress MacNeille in 1990–2002 of the original series, Ashleigh Crystal Hairston in Tiny Toons Looniversity) is a main character of the show.
In Looniversity, Plucky's real name is revealed to be Pluciferous von Mallard Duck and he wears a black shirt instead of white tank tops.
In the series, he seems to have come from a more wealthy background due to having a more snobbish attitude with a less antagonistic and vastly more egotistic behavior and takes cartoon acting more seriously.
Billy West took over the role of Hamton in a few video games after Don Messick's death, including 1999's Tiny Toon Adventures: Toonenstein.
Fifi La Fume (voiced by Kath Soucie in 1990–2002 of the original series, Marieve Herington in Tiny Toons Looniversity) is a young, purple and white female skunk with a pink bow in her hair.
Fifi often unsuccessfully attempts to chase males who have ended up with white stripes painted down their backs, such as Furrball, Calamity Coyote, and Little Beeper.
She can tell fortunes, obtains psychokinetic powers, and is a skilled ballerina; the former hobbies earning her the derisive nickname "Shirley the Loon".
Her design and name are a play on Elmer Fudd; much like her surname, she is also a reversal, having a crushing affection for animals that makes her unintentionally dangerous [5] initially falling into the stereotype of the "spoiled and piercing-voiced girl.
[6] Though initially claimed by Cree Summer to be removed from the show,[7] Elmyra appears in Tiny Toons Looniversity as a photographer obsessed with taking pictures of animals.
[8][9] Montana Max (voiced by Danny Cooksey in 1990–2002 of the original series, Candi Milo in Tiny Toons Looniversity), nicknamed "Monty", is a 12-year-old brown-haired boy and the main antagonist.
Furrball (vocal effects provided by Frank Welker in 1990–2002 of the original series, voiced by Rob Paulsen in "Duck Trek", Natalie Palamides in Tiny Toons Looniversity) is a young, blue male cat with a hole in his right ear and a bandage on his tail.
Furrball is usually depicted as living in a cardboard box in an alleyway, although sometimes he is shown as one of Elmyra's pets and briefly had a home with Mary Melody.
Little Beeper (voiced by Frank Welker in 1990–1995 original series, archive recordings of Paul Julian in Tiny Toons Looniversity) is a young roadrunner with red and orange feathers and blue sneakers.
Much like his Looney Tunes counterpart, the Road Runner, Beeper spends most of his time outrunning and avoiding capture by Calamity Coyote.
Li'l Sneezer (voiced by Kath Soucie in 1990–1995 of the original series, Tessa Netting in Tiny Toons Looniversity), is based on the classic animated mouse Sniffles from the Looney Tunes shorts.
His allergies trigger exaggerated, explosive, gale-forced sneezes capable of seriously damaging or destroying buildings, as Elmyra had the misfortune of finding out.
Arnold has various jobs including lifeguard, doorman, zookeeper, security guard, bouncer, and gym owner and often works for Montana Max in some episodes.
He is also shown to be capable of flight, as seen in the movie Tiny Toon Adventures: How I Spent My Vacation, using his stretchy skin to glide on a waft of air.
She may be based on Granny as she is the human master of Furrball, but she is modeled on the one-shot character So White from the controversial Warner Bros. short "Coal Black and de Sebben Dwarfs".
Mary has a sweet disposition with almost everybody, unlike her human counterparts Montana Max and Elmyra Duff (voiced by Lenore Zann).
Danforth Drake and Margot Mallard (voiced by Rob Paulsen and Kath Soucie), like Roderick and Rhubella Rat, are ducks who are minor characters and students of Perfecto Prep.
It is further confirmed in the "ACME Cable TV" episode that Banjo's family members are inbred and carnivorous with one of his larger relatives proving the latter by devouring Montana Max alive and whole in the show "Possum Swamp Pauper" (though he escapes his stomach during the closing theme shortly after).
Capable of shaping themselves into various instruments from drums to horns, this lively quartet can play almost anything and is usually featured in large or important events, such as the halftime show of the football game in "The Acme Bowl" or the source of the music at the junior prom in "Prom-ise Her Anything".
Lightning Rodriguez is a mouse character who, according to creator Tom Ruegger, was conceived as a Tiny Toon counterpart to Speedy Gonzales.
In the segment itself, villainous wild boar Lord Sebastian tells Lady May (the damsel in distress) that he plans to take her as his wife.
Johnny Pew (voiced by Mark Hamill) is an movie star blue skunk who only appears in Tiny Toon Adventures: How I Spent My Vacation who Fifi has a crush before switched to hate.
Gotcha Grabmore (voiced by Joan Gerber) is an evil businesswoman who kills wild animals to make cosmetics and other stuff.
Dr. Gene Splicer made a movie theater cameo in "How I Spent My Vacation" and appeared in "The Return of Batduck" among the villains that are about to attack the Michael Keaton depiction of Batman before Tim Burton sends stunt actor Plucky Duck in.
Mr. Hitcher (voiced by Rob Paulsen) is a psychotic killer with a hockey mask and chainsaw that Hampton's family and Plucky encounter during their road trip.
In "The Return of Batduck", Mr. Hitcher is among the villains that are about to attack the Michael Keaton depiction of Batman before Tim Burton sends stunt actor Plucky Duck in.