In Brandenburg, Hanseatische Eisenbahn operates local rail passenger transport on the branch lines in the Prignitz.

On 2 November 2012, the Verkehrsverbund Berlin-Brandenburg (VBB) announced that EGP (from which HANS emerged in 2014) would take over these rail connections for (initially) two years from December 2012.

With a reduced offer, the continued operation of the line has been secured since December 2012, with the district of Mecklenburgische Seenplatte now responsible for the task.

Since the timetable change in December 2014, the weekend journeys to and from Meyenburg have been ordered again and VBB tickets are valid without restrictions.

As of the December 2021 timetable change, the Hanseatische Eisenbahn operates five Regionalbahn (RB) routes in Brandenburg, Mecklenburg-Vorpommern, and Saxony-Anhalt.

It also operates the RE 27, a seasonal Saturday-only connection between Bergen auf Rügen and the ferry port at Sassnitz:[3] On behalf of the Putlitz-Pritzwalker-Eisenbahnförderverein (Putlitz-Pritzwalk Railway Promotion Association), school transport was provided on the RB 70 line from Pritzwalk to Putlitz.