It arose comparatively recently, after the beginning of the European Bronze Age, and is mostly prevalent in the population of the Pyrenees region.

Richard A. Rocca made a pioneering study of DF27, which was published in 2012 in the article Discovery of Western European R1b1a2 Y Chromosome Variants in 1000 Genomes Project Data, in the peer-reviewed journal PLOS Biology.

He based his study on 208 people who had tested as R-M269 and were from Great Britain, Tuscany (Italy), Spain, Finland, Utah (United States) and Latin American (Colombia, Puerto Rico, etc).

[2] According to a 2017 article published in Springer Nature entitled, Analysis of the R1b-DF27 haplogroup shows that a large fraction of Iberian Y-chromosome lineages originated recently in situ, DF27 was found in frequences of 40% in the general population of the Iberian Peninsula and in particular spikes at 70% among the Basques.

It is estimated to have developed around 4,200 years ago in north-eastern Prehistoric Iberia as the Neolithic made way for the Atlantic Bronze Age.