Harker's Run drains into Four Mile Creek on the eastern edge of the campus of Miami University in Oxford just north of where the Trenton Oxford Road crosses Four Mile/Talawanda Creek.

The stream flows roughly from north to south, and is approximately 5.5 miles (8.9 km) in total length.

Much of Harker's Run lies within the Bachelor Wildlife and Game Reserve, owned and managed by Miami University (Oxford Township, Butler County, Ohio).

[3] An extensive hiking trail system in the Reserve includes a 45 feet (14 m) long swinging bridge across Harker's Run.

[4] The confluence of this tributary to the larger Four Mile/Talawanda Creek is 0.25 miles (0.40 km) south of the historic Zachariah Price Dewitt Cabin.