The Harn's permanent collection totals more than 11,300 objects, which are focused on Asian, African, modern and contemporary art, as well as photography.

The university sponsors educational programs at the museum including films, lectures, interactive activities, and school and family offerings.

The Cofrin Pavilion features 6,500 feet of exhibition space for international contemporary art, the Camellia Court Café, an outdoor plaza and the Goforth Learning Center, which is used for meetings, programs and educational activities.

On February 6, 2008, Dr. and Mrs. David A. Cofrin made a commitment of $10 million to the University of Florida to fund the addition of a new wing dedicated to Asian art.

[11] The Arts in Medicine (AIM) program at the University of Florida works together with patients to generate new patterns for the zen rock garden as a form of therapy at the Harn Museum.

The Harn's collections include over 10,000 works of art, these are displayed at the museum or other institutions through art-loan programs and traveling exhibits.

[13] The mediums found in this collection range through wood sculpture, textiles, ceramics, leatherwork, beadwork, metalwork, and paintings.

[15] The works in the Contemporary collection ranges from 1945 to the present day, with the mediums included being paintings, photography, multi-media, installation, and film.

There is also a growing collection of Latin American art from Mexico, Chile, Uruguay, Guatemala, Brazil, and Puerto Rico.

The Photography collection ranges from the 19th century to contemporary works, with the mediums included going from daguerreotypes to large-scale color prints.

A strong point for the collection is the works of Jerry Uelsmann, who established the University of Florida as a center for the study of photography.