However, health effects are a function of inhaled materials, of associated behavior changes (notably traditional cigarette smoking), of how and how often the products are used, over what period, manufacturing/distribution quality control, marketing, the regulatory environment, and the actual user population.
[48] TSNAs N-nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosoanabasine, and nitrosoanatabine were detected in five e-liquid samples from two companies at levels comparable to other nicotine replacement products.
[51] The FDA warned several e-cigarette companies for selling e-cartridges and refill solutions containing active pharmaceutical ingredients such as rimonabant (Zimulti) for weight loss purposes and reducing smoking, and tadalafil (for erictile dysfunction).
The pocket may have sufficient moisture to start a chemical reaction within the lithium-ion battery, while the presence of metal objects can create a short-circuit, leading to an explosion.
[100]: Summary, 7 A 2017 review concluded "Exposure to nicotine that was specifically generated by the use of e-cigarettes, was shown to promote oxidative stress and impairment of autophagy, which in turn serves as a potential mechanism leading to development of chronic obstructive pulmonary disease.
[14] A 2017 study reported that vapor containing particulate matter with a diameter of 2.5 μm enters the circulation via the cardiopulmonary system, with a large deposit in the respiratory tract.
E-cigarette and traditional cigarette smoking in individuals with no known cardiovascular disease exhibit similar inhibition of artery dilation in response to the need for more blood flow.
[163] A 2022 study reported that common GI health effects include nausea, vomiting, gastrointestinal discomfort, xerostomia, oral mucositis, gum bleeding, gingivitis, gastric burning, altered bowel habits, and acid reflux.
Vaping is linked to impairment of cognitive processes, increased mood disorders and addiction, damage to functions such as memory, reasoning, impulse control, and attention.
[165] A 202 study reported lower gene expression, reducing occludin, which compromises the stability and strength of the blood-brain-barrier, resulting in neurovascular dysfunction, neuroinflammation, and cognitive defects.
[168]j A 2017 review concluded that nicotine and flavorings may damage periodontal ligaments, stem cells, and gingival fibroblasts in cultures from aldehydes and/or carbonyls from vapor.
[175] : 84 [93] Exhaled vapor consists of mixtures of nicotine, ultrafine particles, primarily propylene glycol, glycerin, flavorings, and aroma transporters,[11] aldehydes,[93] and volatile organic chemicals (VOC)[158] that form a visible fog.
[178] A third reported that vapor may include propylene glycol aerosols at levels that can cause eye and respiratory irritation and exceed California Environmental Protection Agency standards.
A 2017 review concluded that the few studies that examined the effect of indoor air quality on human test subjects in natural settings produced inconsistent results.
The International Union Against Tuberculosis and Lung Disease stated, "Adverse health effects for exposed third parties (second-hand exposure) cannot be excluded because the use of e-cigarettes leads to emission of fine and ultrafine inhalable liquid particles, nicotine and cancer-causing substances into indoor air.
[188] A 2014 WHO report stated passive exposure was a concern, indicating that current evidence is insufficient to determine whether the levels of exhaled vapor are safe to involuntarily exposed bystanders.
Exposure studies suggest that indoor vaping is higher than the smoke-free level put forth by the US Surgeon General and the WHO Framework Convention on Tobacco Control.
In a 15 March 2016, letter to the editor of the Journal of the American Veterinary Medical Association, the Texas Poison Center Network[208] reported 11 cases of dog exposures to e-cigarettes or refills.
[179] A 2016 review concluded, "impurities and nicotine degradation products such as nicotine-cis-N-oxide, nicotine-trans-N-oxide, myosmine, anabasine, and anatabine, which are very carcinogenic, can be reported in e-cigarette refill liquids.
[87] Nicotine promotes endothelial cell migration, proliferation, survival, tube formation, and nitric oxide (NO) production in vitro, mimicking the effect of other angiogenic growth factors.
[219] The Chk2 decrease suggests that nicotine may be capable of overriding DNA damage checkpoint activation, disrupting genetic surveillance, and increasing oncogenesis risks.
[229] A 2017 review concluded that acrolein induces oxidative stress and inflammation, disrupting lung endothelial cell barrier function and may lead to chronic obstructive pulmonary disease.
Common flavoring agents on that list include diacetyl, acetoin, 2,3-pentanedione (buttery), camphor and cyclohexanone (minty), benzaldehyde (cherry or almond), cinnamaldehyde (cinnamon), cresol (leathery) or medicinal (chocolate), and isoamyl acetate (banana).
[237] A 2019 study that sampled e-cigarette delivery systems reported that Juul pods were the only product to demonstrate in vitro cytotoxicity from both nicotine and flavoring chemical content, in particular ethyl maltol.
[75]: 160 Chemicals can be inadvertently produced, especially carbonyls such as formaldehyde, acetaldehyde, acrolein, and glyoxal when the nichrome wire (heating element) reaches a high enough temperature.
[98] The levels of nicotine, TSNAs, aldehydes, metals, volatile organic compounds (VOCs), flavorings, and tobacco alkaloids in e-cigarette vapors vary greatly.
[1] The yield of chemicals reported in the e-cigarette vapor varies depending on, several factors, including the e-liquid contents, puffing rate, and the battery voltage.
[1] The composition of e-liquids varies widely due to the extensive range of nicotine levels and flavoring additives used in these products, which result in a variety of chemical combinations.
[231][12][85] The American Heart Association reported in 2014 that some e-cigarette tank devices with stronger batteries achieve higher temperatures, which may raise nicotine levels.
[174] While myriad studies have been conducted, many questions remain unresolved, including impurities in e-liquids,[100]: Minor Tobacco Alkaloids, 193 effects of nicotine,[217][275] Exposure to e-cigarette components in a susceptible time period of brain development could induce persistent behavioral changes.