The Hopkins River, a perennial river of the Glenelg Hopkins catchment, is located in the Western District of Victoria, Australia.

The Hopkins River rises below Telegraph Hill near Ararat, and flows generally south, joined by twelve tributaries including the Mount Emu Creek, before reaching its mouth and emptying into Bass Strait at Warrnambool.

[6] Together with the Merri River, the Hopkins flows through the regional centre of Warrnambool; and the river passes by the end of the Great Ocean Road near Allansford.

The Hopkins River Re-vegetation Project (HRRP) was commenced in 2016 by the Ellerslie Residents Group Inc.

The river was named in 1836 by Major Thomas Mitchell after a friend, Sir John Paul Hopkins.