Horní Věstonice (German: Oberwisternitz) is a municipality and village in Břeclav District in the South Moravian Region of the Czech Republic.

It has about 500 inhabitants.

Horní Věstonice lies approximately 24 kilometres (15 mi) north-west of Břeclav, 36 km (22 mi) south of Brno, and 209 km (130 mi) south-east of Prague.

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