Horton Creek begins in a pond in Brooklyn Township, Susquehanna County.

It flows south for several tenths of a mile, crossing Pennsylvania Route 167, before turning south-southeast and receiving an unnamed tributary from the right.

The creek then continues flowing south-southeast for more than a mile, passing through a pond and a wetland and receiving an unnamed tributary from the left.

The creek then turns south for several tenths of a mile, passing through two more wetlands and receiving an unnamed tributary from the right.

[3] The average annual rate of precipitation in the watershed of Horton Creek is 35 to 40 inches (89 to 102 cm).

The valley of the creek is relatively narrow and is surrounded by hills that have been rounded due to glaciation.

[4] The channel of Horton Creek is sinuous and flows through glacial drift and a rock formation consisting of sandstone.

[2] The mouth of the creek is in the United States Geological Survey quadrangle of Factoryville.

[12] In the early 1900s, the main industries in the watershed of Horton Creek were quarries, sawmills, and agriculture.

During this time period, the creek was prone to pollution from various causes and typhoid was observed in its watershed.

[14] A concrete stringer/multi-beam or girder bridge carrying State Route 2096 over Horton Creek was constructed in 1940 in Lathrop Township, Susquehanna County and is 26.9 feet (8.2 m) long.

A concrete tee beam bridge carrying State Route 2002 was constructed over the creek in 1961 in Lathrop Township, Susquehanna County and is 35.1 feet (10.7 m) long.

A prestressed box beam or girders bridge carrying State Route 3023 over the creek was built in 1961 in Lathrop Township, Susquehanna County and is 43.0 feet (13.1 m) long.

[15] A prestressed box beam or girders bridge carrying Pennsylvania Route 92 over Horton Creek was built in Nicholson, Wyoming County in 1969 and is 47.9 feet (14.6 m) long.