[2] Trioxidane can be obtained in small, but detectable, amounts in reactions of ozone and hydrogen peroxide, or by the electrolysis of water.

[5] In acetone-d6 at −20 °C, the characteristic 1H NMR signal of trioxidane could be observed at a chemical shift of 13.1 ppm.

[3] Solutions of hydrogen trioxide in diethyl ether can be safely stored at −20 °C for as long as a week.

[6] In 1970-75, Giguère et al. observed infrared and Raman spectra of dilute aqueous solutions of trioxidane.

Recent research found that trioxidane is the active ingredient responsible for the antimicrobial properties of the well known ozone/hydrogen peroxide mix.

Because these two compounds are present in biological systems as well it is argued that an antibody in the human body can generate trioxidane as a powerful oxidant against invading bacteria.