Immacolata was a powerful witch, who was born as a member of the secret race of the Seerkind – mystical beings whose magical abilities have made them the object of fear and hatred by humans.
While an embryo in her mother's uterus, Immacolata manifested her inherent evil for the first time, using the umbilical cords of her triplet sisters in order to strangle them.
She was then forced to abandon the Fugue and started wandering aimlessly to the human world (The Kingdom of Cuckoos) for many centuries (she did not age, thanks to the power of the menstruum).
Even in the human world, Immacolata started gathering a secret circle of fanatics who worshipped her and called her by several aliases, such as Black Madonna, Lady of the Sighs and Mater Maleficorium (mother of evil).
At the same time, she kept having visions of the Scourge, the destructive force that had once almost destroyed the Seerkind, which was now lost in profound slumber, though the possibility of its reawakening filled the Incantatrix with terror.
After all three sisters had expired, they merged into a unified ghost being which haunted the Shrine of Mortalities, an underground crypt storing the bones of those Seerkind who were killed by the Scourge.
The place used to be a favourite hideaway for Immacolata when she was alive and the priests of the church had formed a secret cult, with the purpose of worshipping her.
Eventually, Shadwell and the Scourge (who had possessed Hobart) invaded the church, with the purpose of burning it down, since it was renowned for being a sanctuary of magic.
All three sisters possess unique aspects of sexuality: Immacolata is portrayed as a mysterious and attractive, young-looking woman who persists on sexual abstinence, the Hag is an old and infertile female entity and the Magdalene is sensual and hedonistic as well as a mother, birthing demonic creatures called by-blows created through the rape of human men and used for Immacolata's bidding.
Her most distinguishing aspect is the fact that she wields the menstruum: a glaring and strangely "alive" force that enables her to fly, create a shield around her body, unleash potentially lethal beams and much more, as soon as she concentrates enough to fully manifest it.