Later storylines established him as also being a scientist researching genetics and biochemistry, and revealed an unhealthy romantic obsession he had for Gwen Stacy.

Warren was driven mad with grief and jealousy so he created his Jackal alter-ego to seek revenge on Spider-Man, whom he blamed for Gwen's tragic death.

The character first appears in The Amazing Spider-Man #129 (February 1974), and was created by writer Gerry Conway and artist Ross Andru.

Prior to his Jackal reintroduction, his appearances were essentially limited to the occasional cameo in which he acts as simple background to Spider-Man's civilian life as a college student.

[8] Gwen's death drives Warren into depression, despair, and insanity as a mad geneticist who eventually turns into the Jackal.

Warren continues his research and eventually settles down with Monica, a woman who bears him two children who are all killed in what is originally believed to be a car crash; however, it is later revealed to be an assault by his highly evolved Man-Jackal envious of his creator.

[10] The day after Gwen's death, Warren's lab assistant Anthony Serba reveals that he successfully cloned a frog using their research technology.

Unable to accept responsibility for his actions, Warren develops a second personality to carry the weight of his misdeeds dubbed "The Jackal".

He further develops his alter ego by fashioning a green suit and gauntlets with sharp, claw-like razors on each finger, and by training himself athletically.

[13] Later, he equips wrestler Maxwell Markham with the Grizzly costume and a powerful exoskeleton to assassinate newspaper publisher J. Jonah Jameson.

He then lures his nemesis to Shea Stadium and manipulates into battling his perfect clone of Peter by binding Daily Bugle reporter Ned Leeds to a bomb that only the original Spider-Man can disarm.

[23] He returns in the "Infestation" back-up feature of The Amazing Spider-Man, unleashing genetically engineered bedbugs to pass on Spider-like abilities to thousands of citizens in Manhattan.

[25] Although the bedbugs later die, the virus gives New York's citizens spider-powers and becomes airborne to infect the world and create a new race of Homo-Arachnus as part of his co-conspirator's plan to overtake the Great Web of Life.

Abby-L was manipulated into killing Gwen's other clone, who was living in London under the alias of Joyce Delaney, and coming into conflict with the Jackal and Kaine.

[11] With his own ulterior motives, the Jackal manipulated gang leaders into adorning duplicate costumes of Spider-Man to cause chaos in New York City.

Soria kills him after realizing that his co-conspirator's powers were amplified into the god-like Spider-Queen due to a frequency that returned Spider-Man's spider-sense.

[30] It is later revealed that the Jackal has been monitoring Peter's accidental creation of Alpha, and has set his sights on Spider-Man's new protégé.

[32] When Otto Octavius's mind possesses Spider-Man's body, the X-Men battle a 30 ft. human-spider hybrid attacking New York which turns out to be a human mutant created by the Jackal using Mister Sinister's works, a "daughter" of Scott Summers, Gwen, and Soria named as "Gwen Warren / Spider-Girl".

The ordeal of repeated death caused Ben to become mentally unbalanced and morally ambiguous, due to the trauma and very soul being damaged from being removed and replaced over and over.

Now free with a number of clone of Miles as servants, Ben acts as the new Jackal during the "Dead No More: The Clone Conspiracy" storyline and is determined to repay the people who have heavily influenced Ben's and Peter's lives with the Jackal's technology to make sure that no one has to suffer again and that those who have can become whole, and even does this by establishing New U Technologies.

[38] The Jackal however, survived the fire and targeted the neural net that was built by Dr. Yesenia Rosario when the woman was doing a presentation of it at Empire State University.

[40] He injected himself with actual jackal DNA allowing himself to transform into looking exactly like his iconic green costume, but for real.

[42] The Jackal impersonated Raymond Warren by utilizing his cloning technology to take on his brother's likeness to continue his research without arousing suspicion.

Carrion wielded the power to create a Red Dust that would spread as pestilence as well as his touch that would incapacitate or even cause organic matter to degenerate to the point of disintegration.

"[67] Dan Slott claimed in an interview with Newsarama about the "Spider-Island" saga that the Jackal is "one of the wonderful mad scientists of Spider-Man's world.

The zombiefied Jackal plays an important part in it, creating human clones to feed the remaining Marvel Zombies.

[70] The Ultimate Marvel version of Miles Warren is a hypnotherapist for Harry Osborn to help repress memories about the Green Goblin.

"[76] Spider-Man of Earth-94, Scarlet Spider, and Black Widow of Earth-1610 later encounter Miles Warren when they infiltrate the Baxter Building to disable Jennix' cloning device (which is used to create new bodies for the Inheritors if they get killed in action).

[77] During the Secret Wars storyline, Spider-Gwen encounters the Jackal of Arachnia and covers him with webbing as he is robbing a grave after which he exclaims he is the best geneticist of his generation.

Kaine later told Spider-Man that they have visited various unidentified alternate universes where Peter agreeing to Jackal's plans for New U Technologies have led to catastrophe in the form of the Carrion Virus.