Jamestown is a city in Stutsman County, North Dakota, United States.

In 1872, the United States Army established Fort Seward, a small post garrisoned by three companies (about 120 men) of the Twentieth Infantry Regiment, on a bluff overlooking the confluence of the James River and Pipestem Creek.

The fort only lasted five years, being decommissioned in 1877—but the railroad remained, establishing a repair yard that was among the city's main industries until the 1960s.

Summers can be warm and quite humid, but the winters are very cold with snowfall as early as October.

The top six ancestry groups in the city are German (54.0%), Norwegian (22.4%), Irish (9.0%), English (6.6%), Swedish (4.1%), Russian (3.8%).

Many area families cite their heritage as "Germans from Russia", in reference to ethnic Germans who settled in the Russian Empire in the 18th century, many of whose descendants emigrated to the United States in the late 19th century.

Jamestown has a strong precision manufacturing base as well as food processing, agriculture, retail and wholesale businesses.

Notable companies headquartered in Jamestown include Sunward Steel/Wedgcor Steel Buildings, ACI (Agri-Cover, Inc.), Dura Tech Industries, and Midwestern Machine, and additional major employers include Cavendish Farms and UTC Aerospace Systems.

Service facilities for trucking and heavy equipment repair are also located in Jamestown.

Jamestown Reservoir, a series of three, interlocking, 12-mile-long artificial lakes formed by Jamestown Dam, a flood control a dam on the James River at the north end of the city, is home to watersports and recreational fishing.

The Island Course was the site of the 8th Annual North Dakota Disc Golf Championships in 2007.

After financial hardships, affecting the entire county, Jamestown College had to close its doors in the spring of 1890.

On September 22, 1909, Jamestown College reopened after a population growth in the State due to improved farming methods.

[29] On the northwest side of the city and almost adjacent to the site of historic Fort Seward is The Anne Carlsen Center North Campus[30] (formerly known as the "Crippled Children's School").

In 2024, Anne Carlsen moved operations to the Ballantyne Berg Campus in southwest Jamestown.

A privately funded residential school, it has long been one of the country's leading centers for treatment and education of severely handicapped children.

Jamestown Regional Airport serves the city providing scheduled flights to Devils Lake, North Dakota and Denver, Colorado.

Intercity bus service to the city is provided by Jefferson Lines.