Kamukuzi Division is located in Mbarara Municipality, it covers the northern part of Central Business District.
It is located north of the Central Business District, bordering with Kakoba Division in the East, Nyamitanga Division in the South, Kakiika Division in the North and the West and Nyakayojo Division in the South west.
Kamukuzi Division covers part of the Central Business District with streets of Makhan Singh street, Bwana Victor street, Stanley road and Ntare road.
The division also contains the following neighbourhoods Kamukuzi hill, Ruharo hill, Booma hill and Nkokonjeru hill, all which are upscale residential areas with offices of major Organizations and corporate bodies, Biafra, Andrews, Ntare, Kashanyarazi, Rwebikoona, Kiyanja and Taso Village.
Kamukuzi Division is the 3rd most populous division in Mbarara Municipality after Kakoba and Nyakayojo as of 2014 census report.