The southern terminus of the route is at KY 101 six miles (10 km) north of Scottsville.

The northern terminus is at U.S. Route 68 (US 68) and KY 80 in downtown Bowling Green.

A short distance east of the Bowling Green city limits, KY 234 interchanges with Interstate 65 at exit 26.

[2] In 2002, the junction of KY 234 and I-65 was altered from an overpass to an interchange in order to provide a direct route from the interstate to downtown Bowling Green.

[3] Prior to the opening of the new intersection, the Cemetery Road portion of KY 234 between I-65 and US 31W was widened from two to five lanes to accommodate the anticipated increase in traffic flow.