Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with ISL1 in early motor neuron differentiation and has a suggested role in the ISL1 dependent development of motor neurons.
[1] It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development.
[2] The Drosophila melanogaster protein Chip is required for segmentation and activity of a remote wing margin enhancer.
[3] Chip is a ubiquitous chromosomal factor required for normal expression of diverse genes at many stages of development.
[3] It is suggested that Chip cooperates with different LIM domain proteins and other factors to structurally support remote enhancer-promoter interactions.