Langhorne Creek wine region is a wine region in South Australia that is located on the plains southeast of the town of Strathalbyn along the lower reaches of the Bremer River and Angas River to Lake Alexandrina.

The region received appellation as an Australian Geographical Indication (AGI) in 1998 and as of 2014, has a total planted area of 5,883 ha (14,540 acres) and is represented by at least 24 wineries.

The Langhorne Creek wine region is one of five wine regions forming the Fleurieu zone which is located to the south of Adelaide city centre in South Australia.

The Langhorne Creek wine region extends southeast of the town of Strathalbyn along the Bremer River and Angas River to Lake Alexandrina, centred on the town of Langhorne Creek.

[1] As of 2014, the most common plantings in the Langhorne Creek wine region within a total planted area of 5,883 ha (14,540 acres) was reported as being Shiraz (36.7%) followed by Cabernet Sauvignon (31.6%), Chardonnay (10.6%) and Merlot (6.5%).