The bridge, which originally crossed over Marsh Creek, now spans a dry ditch and is a feature of the Upper Uwchlan Township trail system.

The original bridge was built in 1854 to span Marsh Creek, a tributary of the east branch of the Brandywine Creek, beside Jesse Larkin's Gristmill near the village of Milford Mills, 2.5 miles (4.0 km) southwest of its current location.

In 1972, the Commonwealth of Pennsylvania acquired the Larkin Bridge and relocated it 1 mile (1.6 km) to the north prior to the evacuation of Milford Mills and the creation of Marsh Creek Lake in Marsh Creek State Park.

Larkin Bridge was listed on the National Register of Historic Places in 1980.

Larkin Covered Bridge was rebuilt on the trail path next to the entrance to the Byers Station housing development on Graphite Mine Road near the village of Eagle.