Senators are elected using the first-past-the-post system in single-member constituencies corresponding to the 19 departments of Santa Fe.

In the Chamber of Deputies, 22 members are elected using proportional representation and the D'Hondt method, while the remaining 28 seats are allocated using the majority bonus system.

Much like the Argentine Senate is chaired by the Vice President of Argentina, the Senate of Santa Fe is chaired by the vice governor, who is elected every four years alongside the governor.

[5] Since 2019, Alejandra Rodenas of the Justicialist Party has served as vice governor of Santa Fe.

Both houses of the Legislature meet in the Edificio de la Legislatura, in the provincial capital, Santa Fe.