The product line focuses on Scooby-Doo and the four teenage members of Mystery, Inc. — Fred Jones, Shaggy Rogers, Daphne Blake and Velma Dinkley — arriving at a location in the Mystery Machine, a van painted with psychedelic colors and flower power imagery.

Encountering a ghost, monster, or other ostensibly supernatural creature terrorizing the local populace, they decide to investigate.

Eventually, the kids come to realize the ghost and other paranormal activity is actually an elaborate hoax, and—often with the help of a Rube Goldberg-like trap designed by Fred—they capture the villain and unmask him.

Revealed as a flesh and blood crook trying to cover up crimes by using the ghost story and costume, the criminal is arrested and taken to jail, often with the catchphrase "if it weren't for those pesky kids".

[7][8] As part of the marketing campaign, The Lego Group released five toy sets based on the Scooby-Doo franchise.

Minifigures were released as well, including Scooby-Doo, Shaggy Rogers, Fred Jones, Daphne Blake and Velma Dinkley.

[9][4] Several villains, including Black Knight / Mr. Wickles, Mummy / Dr. Najib, Ghost / Bluestone the Great, Headless Horseman / Elwood Crane,[10] Lighthouse Keeper / Verona Dempsey, Swamp Creature / Mr. Brown[10] and Vampire / Big Bob Oakley to revealed the culprits in the back of its heads[11] except the Zombie / Zeke.

It also included life-size Lego models of Scooby-Doo, Shaggy, Velma, Daphne, Fred and the Mystery Machine in each city.

[21][22][23] In 2015, The Lego Group announced a partnership with Warner Bros. Consumer Products to create a licensing and merchandising programme based on Scooby-Doo franchise, which was released on 1 August 2015.

The Mystery Machine included a driver's cabin with steering wheel, a removable roof and 2 sits for Shaggy and Fred.

Haunted Lighthouse included a balcony, secret skull compartment and underwater rock caves, prison, kitchen with stove and pan and speedboat.

The set included Lego minifigures of Shaggy, Daphne, Lighthouse Keeper / Verona Dempsey, Swamp Monster / Mr Brown and Scooby-Doo.

Mystery Mansion included a clock tower, a greenhouse with a man-eating plant (Venus flytrap), pumpkins, and a kitchen with variety of accessories.

The set included Lego minifigures of Shaggy, Velma, Daphne, Vampire / Bob Oakley, Ghost / Bluestone the Great, Black Knight / Mr. Wickles and Scooby-Doo.

[56] The special premiered on Boomerang channels in the United Kingdom and Ireland in late October 2015 and debuted in Australia and New Zealand on January 26, 2016.

[63][64] The crossover toys-to-life game Lego Dimensions[65][66][67] developed by Traveller's Tales features content based on the original Scooby-Doo.

A "team pack" includes an additional level that recreates the events of the original film and adds Scooby-Doo and Shaggy as a playable characters.

[74] A project based on the Mystery Machine from the Scooby-Doo franchise was uploaded to the Lego Ideas site on December 23, 2020.