Otis (voiced by Kevin James in the film and Chris Hardwick in the series) is a male Friesian cow and the leader of the Barnyard.
Pip's human disguise makes people question him, to which another animal says 'He's the world's smallest man!'.
Pig has a strong, but quite strange, love for unicorns, and he wishes for one in the Christmas special, and is also the animal on the barnyard to dress up and disguise as humans the most times.
Freddy (voiced by Cam Clarke) is a very funny, but dimwitted ferret (although he more closely resembles a waddling kangaroo) who is best friends with Peck, a rooster whom he's often tempted to eat; he used to be a carnivore, but then he's become a vegetarian to assimilate to the barnyard.
Duke (voiced by Dom Irrera) is a sheepdog Border Collie who is in charge of the farmer's flock of intelligent sheep.
Duke only enjoys dog activities, such as playing ball, so he doesn't hang around with Otis and his friends much, and as a result, sometimes feels lonely and left out.
Bessy (voiced by Wanda Sykes) is a sassy and coarse cow that has a tendency to yell and Abby's best friend (Daisy's in the film).
Farmer Buyer (voiced by Fred Tatasciore) is the vegan owner of the farm for whom Otis cares deeply.
Still, when the animals need some time alone to work on a private project (a movie they are trying to make, a theme park they are attempting to build, etc.
Snotty Boy is incredibly dimwitted, mean-spirited, selfish, spoiled, unpleasant, greedy, dishonest, bratty, and obnoxious.
The Sheep (voiced by Chris Hardwick, Leigh-Allyn Baker, Cam Clarke, Rob Paulsen and Jeff Bennett) always hang around together in an eternally-close herd and are more intelligent than Duke.
Etta (voiced by Leigh-Allyn Baker in the series and Andie MacDowell in the film) is the lead hen and the mother of Maddy.
Everett (voiced by Lloyd Sherr) is the farmer's elderly bloodhound who first appears in the film, where he celebrates his 13th birthday, making him 91 in dog years.
Tony Twocheeks (voiced by Rob Paulsen) is a yellow-faced pocket gopher entrepreneur who lives near the farm.
"The Vet" (voiced by Audrey Wasilewski and Julia Sweeney) is a female veterinarian who is called in to give the animals checkups.
Baxter (voiced by Kevin McDonald) is a jack russell terrier who became Duke's nemesis after he met him at an animal clinic in the episode "Doggleganger".
", serves as Santa Claus in "It's an Udderful Life" and hosts the game show "Trivia Cash A-Ding-Dong" in the episode, "A Beautiful Freddy".
Jessica Allspice (voiced by Maria Bamford) is a celebrity pop diva and is featured as a judge on the show Do You Gots It?
Max Fripplehoot (voiced by Maurice LaMarche) is a wealthy man with a hot temper, where he tries to buy Pig's truffle pie recipe.
Bingo (voiced by Mark DeCarlo) is a monkey test pilot who crashes into the farm with his spaceship and he tries to get rid of Otis so he could be Barnyard leader.
He is than defeated by Freddy and it is revealed that the dummy is controlled by termites who want revenge on the animals for destroying their tree home that was used to make it.
Stupid McStinky Head (voiced by Jeff Bennet)' is the name that Snotty Boy gives to his pet Pacific gopher snake.
But when Otis saves his life from a falling anvil, Chubs feels grateful and gives back the barn to the animals.
He first appears in the Barnyard movie, but is never introduced in the television series until the episode "Wild Mike's Dance Party".
In "Paging Dr. Filly", the animals hire him to cure Peck and Freddy's arguing, but he appears to make things worse.
It is then revealed that this wasn't the real Dr. Filly and instead, it is a former patient of his, a donkey apparently named Chip, who likes to impersonate him.
"Weird Al" Yankovic (portrayed as a talking horse and voiced by himself) is Bessy's famous accordion-playing friend.
They appear in "Cow's Night Out", "Treasure Hunt" as the main antagonists, and "A Catfish Called Eddie" as anti-heroes.
Ben has only been mentioned on the show once in the episode "Big Top Barnyard", where Otis says he used to take him to the circus in childhood.
When Ben dies, Miles is crucial in mentoring Otis who helps him assume a position of leadership and defeat the coyotes.