List of Billboard Hot 100 number ones of 1965

These are the Billboard Hot 100 number one hits of 1965.

That year, 14 acts hit number one for the first time: Petula Clark, The Righteous Brothers, Gary Lewis & the Playboys, The Temptations, Freddie and the Dreamers, Wayne Fontana and the Mindbenders, Herman's Hermits, The Four Tops, The Byrds, The Rolling Stones, Sonny & Cher, Barry McGuire, The McCoys, and The Dave Clark Five.

The Beatles, The Supremes, The Byrds, Herman's Hermits and The Rolling Stones were the only acts to have more than one song reach number one that year, with The Beatles having the most with five and The Supremes next with four, and The Byrds, Herman's Hermits, and The Rolling Stones with two.