Jewelpet Twinkle (Japanese: ジュエルペットトゥインクル) is the second Jewelpet anime series created by Sanrio and Sega and animated by Studio Comet, announced in Shogakukan's Pucchigumi magazine and directed by Takashi Yamamoto.

Later, a Jewel Charm appears on her hand and Akari realizes that she was chosen by Ruby to be her partner.

The ending theme titled "Scribbling at the Sky" (空ニラクガキ, Sora ni Rakugaki) by Natsumi Takamori, Ayana Taketatsu, and Azusa Kataoka as Akari, Miria, and Sara respectively.

Nippon Columbia publishes several DVD volumes of Jewelpet Twinkle during the series's airing.

"Fan Discs" were also released due to the show's popularity in Comiket, each containing some episodes and bonus extras.