From 1996 to 1998 Naldini worked in biotechnology in Foster City, California as a visiting scientist and director at Cell Genesys (which was taken over by BioSante Pharmaceuticals in 2009).
At the Vita-Salute San Raffaele University's School of Medicine, he is since 2003 a full professor of cell and tissue biology.
At Milan's San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), he was from 2003 to 2008 the co-director and is since 2008 the director.
[2] Over the last 25 years, he has made significant contribution to the field of gene therapy, from its development to its huge application in the clinic.
[9][10][6] His research also advanced the "use of artificial nucleases for targeted genome editing in cell and gene therapy.