Luis Agote

Agote worked independently and separately from the Belgian surgeon Albert Hustin, who discovered that sodium citrate in tolerable quantities could anticoagulate blood for transfusion on March 27, 1914.

He became Secretary of the National Department of Hygiene in 1894 and became head of the leper hospital in 1895 on the island of Martin Garcia.

In 1667, French scientist Juan Bautista Denys transfused a human with animal blood.

After 6–12 minutes, coagulation begins manifested initially by a gradual increase of viscosity that terminates with almost complete solidification.

Since clots are not created if there is a lack of some of these elements, the addition of sodium citrate (which eliminates calcium ions from blood) prevents its formation.