The Mérida-Los Rosales railway is a Spanish railway line that connects the Extremaduran city of Mérida with Zafra and Los Rosales in Andalusia, a railway junction near to Seville.

The railway line is 204.3km long, it is Iberian gauge (1668mm), non electrified and on a single track.

The concession for the line was given in 1869, and work progressed slowly over the next few years, culminating in 1885.

Services that use the full line are Media Distancia and Regional Exprés services, mostly running from Cáceres to Sevilla Santa Justa.

A daily Seville-Cáceres service continues to Madrid Atocha.