The Macalister River, a perennial river of the West Gippsland catchment, is located in the Alpine and Gippsland regions of the Australian state of Victoria.

The Macalister River rises below Mount Howitt, part of the Great Dividing Range in the southern portion of the Alpine National Park; and flows generally south by east in a highly meandering course.

[5] The fertile flats and valley floor of the Macalister River support agriculture around the town of Licola.

In the Aboriginal Braiakaulung language the river was named Wirnwirndook'yeerun, meaning the "song of some bird",[3] purportedly an emu wren.

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