Manatee Road is a census-designated place (CDP) in Levy County, Florida, United States.

[6] It is bordered to the east by the city of Chiefland and to the northeast by unincorporated Andrews.

Florida State Road 320 (Manatee Springs Road) runs through the southern part of the CDP, leading east to Chiefland and west to its end at Manatee Springs State Park.

According to the United States Census Bureau, the Manatee Road CDP has a total area of 13.0 square miles (33.7 km2), all land.

25.9% of all households were made up of individuals, and 13.4% had someone living alone who was 65 years of age or older.