Provincial Trunk Highway 29 (PTH 29) was a short provincial highway in the Canadian province of Manitoba.

The highway was an at-grade expressway with a length of 0.5 kilometres (0.31 miles) and located within the former Town of Emerson.

It served as a connecting route between PTH 75 and Interstate 29 (I-29) at the Pembina–Emerson Border Crossing on the United States border.

Prior to this, those wishing to travel I-29 were required to turn onto the connecting road north of the border.

After the Noyes and Emerson East border stations were permanently closed in the mid 2000s, the highway alignment remained unchanged until the Manitoba government formally eliminated PTH 29 and moved PTH 75's southern terminus to the Pembina–Emerson border crossing in 2012.