The north-south road lies mostly within the Rural Municipality of Hanover, beginning at PR 311 near New Bothwell and ending at PTH 59 near Rosa.

[1] PR 216 begins in the Rural Municipality of Emerson-Franklin at a junction with PTH 59 just northwest of the community of Rosa.

It now crosses into the Rural Municipality of Hanover, entering the town of Grunthal at a junction with PR 205, with which it becomes concurrent with.

The two overlapped highways head north through neighbourhoods along Froese Road before making a sharp left and going through downtown along Main Street.

PR 216 continues north to pass by Hochstadt shortly before travelling straight through the centre of Kleefeld along Main Street.