Provincial Road 320 (PR 320) is a 18.0-kilometre-long (11.2 mi) north–south highway in the Winnipeg Capital Region of Manitoba, Canada.

It connects the city of Selkirk with Netley Creek Provincial Park.

PR 320 begins in the city of Selkirk at an intersection with PTH 9A (Main Street / Manitoba Avenue) in downtown.

It heads north along Main Street as four-lane divided highway for several blocks through neighbourhoods before narrowing to a two-lane at an intersection with Lake Avenue.

PR 320 continues along the banks of the Red River as it passes through suburban areas for the next several kilometres, with pavement ending at the Clandeboye Road intersection, where it transitions to a gravel road.