In 1994, she entered the Czech Ministry of Foreign Affairs and worked for one year at the Department of Analysis and Planning.

In 1999, she came back to Prague and served as Head of Unit within the Department of the Coordination of EU Relations.

In 2013, she became the Permanent Delegate of the Czech Republic to UNESCO (2013-2016), and was elected as Chair of the organization's Committee on Conventions and Recommendations (2013-2015).

In July 2017, she was elected as the 73rd President of the Economic and Social Council of the United Nations (ECOSOC).

[6] Aside from her own career, Chatardova has held teaching positions at the Faculty of Social Sciences of Charles University (Institute of Western European studies), Anglo-American University (School of International Relations and Diplomacy), the Institute of Public Administration in Prague, and the Diplomatic Academy in Prague.