She was the first plant pathologist at Rothamsted Experimental Station and was particularly interested in soil-based fungal diseases including potato wart, eyespot in wheat and take-all.

[1] As soon as Glynne graduated she was offered a post in the University College of North Wales department of agriculture, but within a few months she was volunteering at Rothamsted Experimental Station where E. John Russell was director.

Winifred Brenchley, the first woman to work in agricultural sciences, soon offered her a permanent assistant botanist position at Rothamsted,[1] where she remained for 43 years.

[2] Glynne was the British winner of the Dr. Georgina Sweet fellowship in 1927,[1] awarded at the Vienna conference of International Federation of University Women.

She was able to prove that the lodging or flattening of wheat close to harvest was frequently not the result of wind or rain, but instead caused by take-all and eyespot, both soil-borne fungal diseases.

[1] Glynne's work on crop diseases was subject to significant attention towards the end of World War II, as maximising the production of food was essential due to shortages.

[2] In both the 1940s and 1950s, she carried on her pioneering work in cereal pathology[6] and made "unexpected" discoveries related to the disposal of organic material in the soil.

[3] When she returned to Europe, she climbed major Alpine peaks such as Matterhorn, Mont Blanc, La Meije and Aiguille du Dru in the 1930s.

[11] Glynne was made a fellow of the Institute of Biology[1] and in the 1960 Birthday Honours, she was appointed Officer of the Most Excellent Order of the British Empire (OBE) for her services to agriculture,[12] and retired that same year.