Years later the track would be remixed for the webcomic Homestuck's soundtrack as "MeGaLoVania" with help from Joren "Tensei" de Bruin, and Fox would use another rendition as the final boss theme for the "genocide" route in his 2015 video game, Undertale, when fighting the character Sans.

In 2008, starmen.net, a fansite dedicated to the Mother video game series, held its annual "Halloween Funfest" competition for fan works related to the franchise.

[9] Meanwhile, the melody itself was inspired by the final boss theme of Brandish 2: The Planet Buster, a game series that heavily influenced several other aspects of the ROM hack.

[9] Toby Fox later worked as a musician for the webcomic Homestuck, and in 2011 "Megalovania" was remixed for its sixth volume, Heir Transparent with Joren "Tensei" de Bruin providing guitar music to the track.

After its release, despite both he and de Bruin being happy with the final results he acknowledged some of the criticisms it had received, stating he felt the guitar solo he had added was "uncreative" and would do the track over without the Super Nintendo audio for later renditions.

[20] The Twitter account for Cult of the Lamb utilized the song in promotional material, with the social media handler using the game's merchandise to sound out "Megalovania"'s notes and encourage sales of it.

[18] For Pesterquest, a game set in the Homestuck universe, musician James Roach utilized the melody from "Megalovania" for the track "Yeah It Is".

[22] Polygon's Palmer Haasch described the song as having "consistent presence in internet game culture of the late 2010s", which he attributed in part due to Undertale's success and its frequent use in fanmade remixes and "shitposts" that persisted for years,[23] the former of which had formed its own subgenre on YouTube according to Jen Glennon of Inverse.

[24] Haasch also noted the song's massive presence on the social media website TikTok, stating that "its repetitive riffs and melodramatic, chiptune-esque palette ... play out well on TikTok because they make sounds memorable and quirky", and worked well with the various meme cultures already forming on the platform, and adaptions of older memes such as rickrolling to incorporate the song.