The literature specifically demonstrates the use of MDS in sizing protein-nanobody complexes, monitoring the formation of α-synuclein amyloid fibrils.
Since the detection is not based on inherent fluorescence of tryptophan or tyrosine residues, MDS has been used as an alternative to A280 UV-Vis quantification.
[9] Another key advantage is that results can be obtained with very small quantities of material[10] which may be particularly important where samples are scarce or expensive.
Finally the method does not require calibration, as it relies on a ratio-metric measurement to determine diffusion rate.
The number of particles in each stream can then be detected (in the case of proteins this is achieved by addition of an amine reactive fluorogenic dye).