The club was founded in 2000 and currently plays in the Women's Premiership[1] and at the Mid Ulster Sports Arena.
Future Northern Ireland Women's Football Association chairwoman Elaine Junk, who was playing for Dungannon Athletic in 2000 decided to found Mid-Ulster Ladies F.C.
[3] Following a reorganisation of women's football in Northern Ireland, in 2004 Mid-Ulster Ladies were placed into the Mid Ulster League.
[6] In 2013 Mid-Ulster Ladies finished in the promotion-relegation playoff place, where they would compete against NIWFA Division 2 runner-up Cliftonville Ladies however they managed to win and retain their Women's Premier League status before making the final of the Premier Cup, where they were defeated 2-0 by Glentoran Belfast United.
Mid-Ulster Ladies retained their Women's Premier League place again by winning 8-3 on aggregate.