In the 2nd Century CE, the Roman 10th Legion based at Vindobona built an extensive outpost near the Amber Road and the Pálava Hills in Mikulovská, near the present-day village of Pasohlávky.
[citation needed] Modern-day archaeological excavations of the ancient Roman outpost near Pasohlávky have yielded many artifacts, including a vine pruning knife.
[2] Viticulture was practiced during the Great Moravian Empire (833–906), as evidenced by numerous pruning knives and grape seeds unearthed during archaeological excavations of Slavic settlements.
[3] In 1249, Ottokar II of Bohemia granted the land of Mikulov (then called Nikolsburg) and the surrounding area to the Austrian noble Henry I of Liechtenstein.
In 1309, a new set of viticultural and winemaking regulations was established for many southern Moravian villages, based on the application of Bergrecht laws and vineyard rules of Falkenstein, Lower Austria in the district of Mikulov.
In 1763, Austrian vintners asked Maria Theresa to limit new vineyard plantings in Moravia to reduce the competition from Moravian wines.
The vine pest Phylloxera arrived in Mikulov and Dolní Dunajovice in 1900, struck Perná in 1901, and hit Horní Věstonice and Bavory in 1902, devastating the vineyards.
The rolling Milovická Hills, located east of Mikulov, are composed of Mesozoic limestone and Cenozoic deposits of flysch argillites and sandstones.
The Thaya (Czech: Dyje) River runs through the region from west to east, with a series of three dams forming the three Nové Mlýny reservoirs.
Thirty wine municipalities, along with 182 registered cadastral vineyard tracts, are situated within Mikulovská and are defined under the Czech Viticulture Act.