The route runs for about 2.126 miles (3.421 km) under the monikers of Stockyards Expressway and South Second Street beginning at an intersection with West Florence Road.

The southern terminus of Route 759 is at an at-grade intersection with Southwest Lower Lake Road in the stockyards section of the city.

Route 759 begins at an intersection with Southwest Lower Lake Road and Packers Avenue in the stockyards section of St. Joseph.

After the intersection with West Florence Road, Route 759 changes monikers from Stockyards Expressway to South Second Street.

At that point, the route makes a gradual bend to the north along with the nearby Missouri River, becoming Stockyards Expressway once again as it parallels I-229 and US 59.