Mitchler Run is a tributary of Huntington Creek in Luzerne County, Pennsylvania, in the United States.

[1] Mitchler Run joins Huntington Creek 23.5 miles (37.8 km) upstream of its mouth.

[2] Mitchler Run is entirely in the United States Geological Survey quadrangle of Sweet Valley.

[3] Mitchler Run is considered by the Pennsylvania Fish and Boat Commission to be Class A Wild Trout Waters between its headwaters and its mouth.

The stream is one of six direct tributaries of Huntington Creek to be designated as Class A Wild Trout Waters.