The basin of the Molenbeek is located in the province of East Flanders, and flows through the municipalities of Wichelen (Schellebelle, Serskamp, Wichelen), Lede (Wanzele, Impe, Smetlede, Papegem, Lede, and Oordegem), Erpe-Mere (Erondegem, Vlekkem, Ottergem, Bambrugge, Egem, and Burst) and Herzele (Borsbeke, Herzele, Ressegem, and Hillegem).

The south of the basin comprises a small part of the Zottegem municipality (Grotenberge and Leeuwergem).

In the west, it crosses the border of the Sint-Lievens-Houtem territory (Vlierzele, Zonnegem, Letterhoutem).

The Molenbeek (millbrook) is part of the Drie Molenbeken drainage basin.

From its source in Grotenberge to the delta nearby, Wichelen of the Molenbeek has the following tributaries: Valleibeek, Fonteinbeek, Doormansbeek, Kasteelgracht, Hellegat, Smoorbeek, Kokelaarsbeek, Zijpbeek, Trotgracht, Overimpebeek, Beekveldzijp, Wellebeek, and Vijverbeek.