Molfetta Cathedral, otherwise the Church of the Assumption and of Saint Ignatius Loyola (Italian: Duomo di Molfetta, Cattedrale or Chiesa di Santa Maria Assunta e Sant'Ignazio di Loyola), is a Roman Catholic cathedral in Molfetta (the "new cathedral"), dedicated to the Assumption of the Virgin Mary and Saint Ignatius Loyola.

Originally a Jesuit church, it became the seat of the bishops of Molfetta in the late 18th century.

The present cathedral was built by the Jesuits during the 17th century and dedicated to their founder, Saint Ignatius Loyola.

Among the works of art in the cathedral are the Dormitio Mariae ("Dormition of Mary") attributed to Scacco (16th century), the monument of the Molfettese naturalist and historian Giuseppe Maria Giovene, to the left of the altar dedicated to Saint Conrad, and on it, the magnificent canvas by Corrado Giaquinto of the Assumption of the Virgin Mary.

In 1887 the ceiling vault was decorated in tempera by the Molfettese painter Michele Romano.