According to some historians, Monterotondo is the heir of the ancient Sabine town of Eretum, although the modern settlement appeared in the 10th-11th centuries in a different location.

In the Middle Ages, due to its location across the Via Salaria, Monterotondo was a strategic point for the defense of Rome.

It has become established as a basic research center of excellence and innovation in mouse genetics and functional genomics.

However, the club started to play in Rome and was renamed Polisportiva Monterotondo Lupa in 2011 and again relocated to Maccarese, Fiumicino as Pol.

Another team of the city, Atletico Monterotondo, merged with another football club from Marino and started to play in Eccellenza Lazio in 2013 as A.S.D.