It is the northern terminus of Ontario Northland's Polar Bear Express, located about 12 miles (19 km) south of the shore of James Bay on the north bank of the Moose River.

[4] As of April 2021, the train operates four days per week, with an afternoon arrival and early evening departure on Monday, Tuesday, Thursday, and Friday.

Tracks run past the station out to the Moosonee Airport with a siding along the Moose River (beside Revillon Road North) for bringing freight to barges.

[6][7] Box cars are stored at the terminus until trains run south for the return trip to North Bay or Toronto.

[citation needed] The MV Niska I passenger-vehicle and freight ferry operated by the Owen Sound Transportation Company connects Moosonee to Moose Factory across the river on a seasonal basis.